The recent paper “Antibody Validation for Western blot: By the User, for the User” is an essential guide for validating primary antibody specificity, selectivity, and reproducibility in Western blotting. This resource reflects a four-year collaboration between LI-COR and Abcam; together, they equip you with validation strategies that easily suit many research platforms and help you achieve success in the lab. This comprehensive guide explains how you can validate primary antibodies for your research needs. Take an in-depth look at why primary antibodies should be validated and how it can help you achieve accurate, publishable data. Otherwise, variation in assay methodology can complicate or prevent reproducibility. This may even hinder data publication due to increasingly stringent journal guidelines. Following the paper’s easy steps can help standardize your practices for publishable results.
In addition to useful steps for successful publication, you can use this paper is a practical resource for your entire lab. Tables full of antibody search engines and online databases, necessary reporting details, and categories for validating antibody performance all serve as an essential reference source to keep on hand. Its comprehensive collection of figures includes practical examples of different detection methods, such as epitope-tagged antibodies and multiplexed Western blots. This valuable information can help guide your research practices for useful results.
Moreover, this primary antibody validation paper also reminds us that collaboration and cooperation among users, vendors, and publishers are vital for useful successful data. Vendors are an asset to your research, supplying important validation data and quality primary antibodies. Publishers help maintain standards and give you open access to others’ experimental methods. Users, meanwhile, are responsible for adopting and recording replicable steps. Together, these three groups help facilitate reproducibility and help improve our scientific community as a whole.
“Antibody Validation for Western blot: By the User, for the User” provides strategies and recommendations for validating primary antibodies. It explains why validating primary antibodies is important; it then shows you how to validate your own research methods using easy-to-follow steps.
Keep its tables and figures nearby to encourage new ideas and success on your lab bench. Finally, use vendors and publishers as a resource for producing usable data. Altogether, this comprehensive paper is aptly named for its ability to convey imperative information from one scientist to another and help promote antibody standardization, reproducibility, and performance all in one place.
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