Qualitative Western Blot Overview
Qualitative Western Blot Experiments provide quick signal identification data and charts.
Qualitative Western Blot Experiments are similar to Quantitative Western Blot Experiments, but Quantitative Western Blot Experiments include additional validation Experiments and provide additional calculations that you may not need for quick signal identification.
Summary of Differences between Qualitative and Quantitative Western Blot Experiments
This table highlights some key differences between Qualitative and Quantitative Western Blot Experiments.
| Qualitative Western Blot | Quantitative Western Blot | |
|---|---|---|
| Antibody Validation | ||
| Linear Range Determination | ||
| Housekeeping Protein Validation | ||
| Warnings on Target Analysis Starting Pages for Incomplete Validation Experiments | ||
| Set up calculations step in Target Analysis (used to setup fold change calculations, etc.) |
Overview of Qualitative Western Blot Experiments
| Experiment Type | Purpose | Process | Detection | Maximum Images | Maximum Targets |
|---|---|---|---|---|---|
| Antibody Validation | Validate primary antibodies for use in your intended assay and sample context. | Evaluate antibodies in your assay to confirm expected performance. | Fluorescence, chemiluminescence | 3 | 4 |
| Analysis: TPS + Targets | Compare target bands to total protein. | Quantify and compare the total protein and target signals in the chosen blot images. | Fluorescence | 3 | 4 |
| Qualitative Analysis: Target Proteins | Compare target protein bands. | Quantify and compare the target protein band signals in your chosen image(s). | Fluorescence, chemiluminescence | 3 | 4 |