What's New in Image Studio™ Software

Release Notes

Image Studio 6.0 introduces support in Image Studio for acquisition and analysis with the Odyssey^® M Imager and the Odyssey F Imager.

Specific changes include:

Support has been added for image acquisition with the Odyssey M and analysis of images from the Odyssey M, including:
1. New functionality for processing Epi and Trans images and support for RGB images
2. Tools to enable easily replicable scans with the plate alignment guide and slide holder
Support has been added for image acquisition with the Odyssey F and analysis of images from the Odyssey F, including:
1. Tools to enable easily replicable scans with the plate alignment guide and slide holder
New menu options are available for scanning with the plate alignment guide (926-18972) and slide holder (926-18971). The Odyssey M and Odyssey F use the same plate alignment guide (called “plate holder” for short in the software) and slide holder.
The new menu has three options. The three options are outlined below. For more detail on the three options, see the Odyssey® M Imager Acquire Tab topic or the Odyssey® F Imager Acquire Tab topic.
- No Holder - Choose No Holder to see an open canvas for drawing scan areas anywhere on the scan surface. This option matches the functionality for Odyssey Imagers in prior versions of Image Studio, and is great for scanning membrane and gel assays.
- Plate Holder - Choose Plate Holder to display a view with four plate areas. Select the plate area(s) that you want to scan.
  LICORbio recommends using the plate alignment guide (926-18972) and the Plate Holder option when imaging plates on the Odyssey M and Odyssey F to help with consistent sample placement and scanning.
- Slide Holder - Choose Slide Holder to display a view with twelve slide areas on which you can draw scan areas.
  LICORbio recommends using the slide holder (926-18971) and the Slide Holder option when imaging slides on the Odyssey M and Odyssey F to help with consistent sample placement and scanning.
Added support for RGB images from the Odyssey M, including editing (crop, rotate, etc.), lookup table display adjustment, and lab book reports.
For third-party color TIFF imports, Image Studio 6.0 supports 8-bit, RGB color TIFFs. Attempting to import other third-party color TIFF images may not succeed, and a warning message with the text "Error Adding Acquisition" may be displayed.
Added an Invert button on the Image tab that inverts U16 (non-RGB Epi and Trans) images. The Invert option is intended for use on images where the sample region that you intend to quantify has lower pixel intensity than the image’s background (e.g., plate assays imaged in Trans channels).
Removed functionality that automatically inverted images on import based on a heuristic. The new Invert button allows the user to decide when to invert an image.
Updated the import feature to support RGB, Epi, and Trans images from the Odyssey M.
- To transfer image acquisitions from LI-COR Acquisition Software to Image Studio, use the Export for Analysis option in LI-COR Acquisition software and the LI-COR Zip Files import option in Image Studio.
- To transfer image acquisitions from Empiria Studio Software to Image Studio, use the Export for Analysis option in Empiria Studio software and the LI-COR Zip Files import option in Image Studio.
Default color choices for fluorescent channels have been set based on existing Image Studio conventions and to distinguish expanded channel selection. Color selections can be changed per acquisition in the Display panel and defaults can be changed in Application menu > Preferences > Image Display.
- 488: White-on-Black
- 520: Blue
- 700: Red
- 800: Green
Added support for image transformations (such as image rotation) on Epi and Trans images.
Note: Rotating Epi and Trans images works differently than rotating fluorescent images. If an Epi or Trans image is free-rotated to an angle that is not a multiple of 90 degrees, then "filler pixels" must be added to the image for the image to remain a rectangle. In fluorescent and chemiluminescent images, these filler pixels have the half-float value "NaN" (not a number) to indicate that the filler pixels have no quantification meaning. Epi and Trans images use integer values instead of half-float, so filler pixels added to an Epi or Trans image have the integer value "0". Because the filler pixels have a quantifiable value, be careful not to accidentally include filler pixels in shapes when you are quantifying regions of your Epi or Trans image.
If you do need to invert an Epi or Trans image, be careful to Invert the image as soon as you can after importing the image. If you rotate an Epi or Trans image and then invert, the filler pixels will be set to the maximum allowable integer value instead of the lowest allowable integer value (i.e., 0).
Updated the dialog for importing acquisition zip files to filter files by the .zip file extension. This makes it easier to find the zip file to import.
Added detailed tooltips to channel selection options on the Acquisition ribbon, similar to the tooltips over channel selections in the Select Detection Channel dialog in LI-COR Acquisition Software.
Improved image metadata transfer for when images are imported from LI-COR Acquisition Software or when images from Image Studio are imported into Empiria Studio. The following image data columns from LI-COR Acquisition software to the Image Studio Image Table: Sample Up/Down (for slides), Scan Type, Specimen Type, Scan Position, Plate Well Format, and Biomarker.
Raised the number of channels that can be selected for an image acquisition on the Odyssey M to 6 channels (compared to 4 in LI-COR Acquisition Software).
Changed default export and import folder location to the user’s home directory instead of the location of the current Work Area.
Enhanced functionality for importing settings from existing Work Areas into the new Work Area when creating a new Work Area. The option to Import Settings from one Work Area to another is now located in the Create New Work Area dialog.
Added functionality to recalculate the Z′-Factor value shown on the In-Cell Western^™ Analysis tab when the plate grid is moved and resized. (The Z′-Factor value exported in the Lab Book report was already updated after move/resize in previous Image Studio versions.)

Other Changes in Image Studio 6.0:

Set the Date/Time column of the Status Message History dialog to a fixed width that shows the full date/time, which enables you to expand the whole dialog to show more of the Status Message column without having to then reduce the width of the Date/Time column. A horizontal scroll bar has also been added to make it easier to read status messages.
The Desktop and user.home directories are now recorded in the log files. This helps diagnose problems caused by Desktop or user.home directories being mapped to network drive locations or syncing folder locations (like OneDrive). Image Studio supports local Work Areas, not Work Areas set on a network drive or syncing cloud storage location.
Updated the Application button > Export Zip Application Log File functionality so that the export location can be chosen instead of placed only on the Desktop.
Removed outdated references to Connections Diagnostic utility.
The RSS feature no longer reaches out to the RSS feed to get more information. Click the RSS button to view more Image Studio information on the LICORbio website.
Added support for the Norsk negative sign in PDF exports. In previous versions of Image Studio, the negative sign was not shown in PDF exports.
Improved the Set Active Work Area dialog (the dialog that appears when Image Studio first starts) so that it appears over top of other open windows on the computer, which makes it much easier to find.
Upgraded to Java version 17.0.8.
Upgraded some third-party libraries (third-party libraries are listed at https://opensource.licor.com/licenses/bio/index.html).

Known Issues

Summary

Details

Solutions

The Focus Position option for the Pearl Imager does not respond when clicked.

The Focus Position button is located at the bottom of the Focus Position list on the Acquire ribbon for the Pearl Imager. The Focus Position option automatically acquires images at different focus offsets to help in determining the best focus position to use for a particular sample.

In Image Studio 6.0, the Focus Position button does not respond when clicked.

You can acquire images at each focus position that you want to test without using the automatic Focus Position option. For additional information and help with questions, please contact biohelp@licor.com.