Protocols and Webinars for Developing In-Cell Western Assays
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In-Cell Western
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In-Cell Western
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In-Cell Western
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Assay Resources
Resources
Handbooks
In-Cell Western™ Assay Development Handbook
Application Guides
In-Cell Western™ Assay: FAQs for Suspension/Non-Adherent Cells Application Guide
Cell Viability Assay with Sapphire700™Stain and the Odyssey® CLx and Sa Imaging Systems Application Guide
Brochures
Solutions for Advanced Scientific Research Brochure
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Developing Targeted Therapeutics Brochure
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Quantitative methods for analysis of protein phosphorylation in drug development white paper
A cell-based immunocytochemical assay for monitoring kinase signaling pathways and drug efficacy white paper
Protocols
Probe Design and
in vitro
Validation Protocol
Example Experiments
Detecting Feline Panleukopenia Virus in CrFK Cells Using Virus Titration Example Experiment
Measuring Apoptosis in HeLa Cells Following Anisomycin Treatment Example Experiment
Detecting p38 Activation in HeLa Cells Following Anisomycin Treatment Example Experiment
Detecting p53 Activation in COS-7 Cells Following Hydroxyurea Treatment Example Experiment
Characterizing AKT Activation in Response to PDGF-BB Treatment in NIH-3T3 Cells Example Experiment
Characterizing ERK Activation in Response to EGF Treatment in A431 Cells Example Experiment
Characterizing ERK Activation in Response to EGF Treatment in HeLa Cells Example Experiment
Characterizing ERK Activation in Response to FGF Treatment in NIH-3T3 Cells Example Experiment
Characterizing U0126 Inhibition of the FGFR Signaling Pathway in NIH-3T3 Cells Example Experiment
Characterizing ERK Activation in Response to Phorbol 12-Myristate 13-Acetate (PMA) Example Experiment
Webinars
Developing Targeted Therapeutics: What You Need to Know
See some published examples of In-Cell Western Assays
View publications