IRDye® 700 AP-1 Consensus Oligonucleotide
IRDye® 700 CREB Consensus Oligonucleotide
IRDye® 700 HIF-1 Consensus Oligonucleotide
IRDye® 700 NFĸB Consensus Oligonucleotide
IRDye® 700 p53 Consensus Oligonucleotide
IRDye® 700 Sp-1 Consensus Oligonucleotide
IRDye® 700 STAT3 Consensus Oligonucleotide
A DNA oligonucleotide end-labeled with IRDye 700 is a good substrate for protein binding. IRDye DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol.
Additional benefits include no hazardous radioisotopes, no gel transfer to membrane or gel drying, no chemiluminescent substrate reagents, and no film exposure. Following electrophoresis, the gel can be imaged while in the glass plates. If necessary, the gel can be placed back in the electrophoresis unit and run longer.
Existing mobility shift assay protocols can be easily transformed into infrared assays by replacing the existing DNA oligonucleotides with IRDye oligonucleotides. The binding conditions and electrophoresis conditions will remain the same as with any other EMSA detection method.