10X Orange Loading Dye is used as a loading buffer for DNA gel electrophoresis. This loading dye is not visualized when imaged on Odyssey® Imagers. The primary application for 10X Orange Loading Dye is for electrophoretic mobility shift assays (EMSA). When run on a 5% native acrylamide 1X TBE gel, the orange dye migrates with the 20-28 bp DNA fragment.