IRDye 680RD NHS Ester Used to Label Primary or Secondary Amines.

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

IRDye® 680RD NHS Ester reaction — NHS Ester reacts with a primary aliphatic amine, such as lysine.

IRDye® 680RD NHS Ester amide bond — Dye label is attached through amide bond.

Which 700 Channel Dye Should I Use?

Dye Structure

Properties

Chemical Formula: C₄₃H₄₅CIN₄Na₂O₁₃S₃
Molecular Weight: 1003.46 g/mol
Exact Mass: 1002.16

Absorption and Emission Spectra

Solvent	Ext. Coeff. (M^-1cm^-1)	Absorption Maxima	Emission Maxima
Methanol	170,000	680 nm	694 nm
1X PBS	165,000	672 nm	694 nm
PBS: Methanol	165,000

Absorption and Emission Spectra in 1X PBS

IRDye 680RD Absorption and Emission Spectra

IRDye® 680RD NHS Ester

Dye Structure

Properties

Absorption and Emission Spectra

Absorption and Emission Spectra in 1X PBS

IRDye^® 680RD NHS Ester