IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.
Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.
Dye Structure
Properties
- Chemical Formula: C50H54N3Na3O17S4
- Molecular Weight: 1166.20 g/mol
- Exact Mass: 1165.20
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima |
|---|---|---|---|
| Methanol | 300,000 | 778 nm | 794 nm |
| Water | 240,000 | 774 nm | 789 nm |
| 1X PBS | 240,000 | 774 nm | 789 nm |
| PBS: Methanol | 270,000 | 777 nm | 791 nm |
Absorption and Emission Spectra in 1X PBS
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