Immunogen
Mouse IgG paraproteins
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
Applications
Highly recommended for:
- Western Blots
- In-Cell Western™ Assays
- On-Cell Western Assays
- Protein Arrays
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
Formulation
IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Recommended Dilutions
Application | Recommended | Suggested Range |
---|---|---|
Odyssey Western blot detection | 1:20,000 | 1:5,000 - 1:25,000 |
In-Cell Western Assay detection | 1:800 | 1:200 - 1:1,200 |
Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-32210: RRID AB_2687825
- P/N 926-32210: RRID AB_621842