Label Primary and Secondary Amines with IRDye 680LT Infrared Dye.

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

Which 700 Channel Dye Should I Use?

Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

IRDye® 800CW NHS Ester reaction — NHS Ester reacts with a primary aliphatic amine, such as lysine.

IRDye® 800CW NHS Ester amide bond — Dye label is attached through amide bond.

Dye Structure

Properties

Chemical Formula: C₅₄H₅₂N₃Na₃O₂₂S₆
Molecular Weight: 1402.32 g/mol
Exact Mass: 1401.09

Absorption and Emission Spectra

Solvent	Ext. Coeff. (M^-1cm^-1)	Absorption Maxima	Emission Maxima
Methanol	250,000	680 nm	694 nm
1X PBS	250,000	676 nm	693 nm

Absorption and Emission Spectra in 1X PBS

IRDye® 680LT Absorption and Emission Spectra

IRDye® 680LT NHS Ester

Dye Structure

Properties

Absorption and Emission Spectra

Absorption and Emission Spectra in 1X PBS

IRDye^® 680LT NHS Ester