IRDye 800CW Goat anti-Mouse IgG2b-Specific Secondary Antibody.

Immunogen

Mouse IgG_2b paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG_2b covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG_2b. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG₁, IgG_2a, IgG₃, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ Assay applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

Applications

Highly recommended for:

Western Blot
In-Cell Western Assay
On-Cell Western Assay
Protein Array
Immunohistochemistry
Small Animal Imaging
Microscopy
2D Gel Detection
Tissue Section Imaging
Virus Titration Assay

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:5,000 - 1:25,000
In-Cell Western Assay detection	1:800	1:200 - 1:1,200
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

P/N 926-32352: RRID AB_2782999

Example Data

IRDye® 800CW Goat anti-Mouse IgG2b Specific Secondary Antibody data — Purified mouse subclasses were detected by Western blot with IRDye 800CW Goat anti-Mouse IgG_2b (P/N 926-32352) to demonstrate that it detects the heavy chains of mouse IgG and mouse IgG_2b.

IRDye® 800CW Goat anti-Mouse IgG2b-Specific Secondary Antibody