CellTag 700 Stain for Accurate Normalization in ICW Assays.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

CellTag 700 Stain is detected in the 700 nm channel of Odyssey^® CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye^® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.

Properties

Size: 2 x 10 nmole (lyophilized)
λ_Ex/λ_Em: 675/697 nm
Recommended concentration: 0.2 μM

Linear Relationship Between Fluorescence and Cell Number

**Two-fold serial dilutions of A431 and NIH/3T3 cells were plated in 96-well plates.** Cells were fixed, permeabilized, stained with CellTag 700 Stain (0.2 μM), and detected with the Odyssey Classic Infrared Imaging System. Trim Signal values were used to generate the graphs.

CellTag™ 700 Stain for In-Cell Western™ Assays

Properties

Linear Relationship Between Fluorescence and Cell Number