IRDye 680RD Goat anti-Mouse IgG Secondary Antibody

IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody

IRDye 680RD Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western Assay applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Formulation

IRDye 680RD Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:20,000 1:5,000 - 1:25,000
In-Cell Western Assay detection 1:800 1:200 - 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-68070: RRID AB_2651128
  • P/N 926-68070: RRID AB_10956588

Selected P/N: 926-68070,925-68070

Price

Quantity

Add to Cart

+ Add to WishlistAdded. Go to Wishlist

Generate an Autoquote

*Includes all LICORBio secondary antibodies
Frequently bought together: