IRDye 750 Maleimide is an infrared dye with detection near 750 nm. Maleimide reactive group provides the functionality for labeling molecules that contain free sulfhydryl (–SH thiol) groups. This reactive group allows conjugation reactions to be performed at physiological pH.
This dye is not suitable for use with LI-COR Odyssey® Imaging Systems.
Dye Structure
Properties
- Chemical Formula: C55H63N4Na3O15S4
- Molecular Weight: 1217.33 g/mol
- Exact Mass: 1216.29
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima |
|---|---|---|---|
| Methanol | 330,000 | 766 nm | 776 nm |
| 1X PBS | 260,000 | 756 nm | 776 nm |
| PBS: Methanol | 260,000 |
Absorption and Emission Spectra in 1X PBS
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