IRDye 800CW Donkey anti-Rabbit IgG Secondary Antibody

IRDye® 800CW Donkey anti-Rabbit IgG Secondary Antibody

IRDye 800CW Donkey anti-Rabbit IgG Secondary Antibody

Immunogen

Rabbit IgG

Purity and Specificity

The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains common to most rabbit immunoglobulins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western Assay applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • FRET-Based Assays
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:5,000 - 1:25,000
In-Cell Western Assay detection 1:800 1:200 - 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32213: RRID AB_2715510
  • P/N 926-32213: RRID AB_621848

Selected P/N: 926-32213,925-32213

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*Includes all LICORBio secondary antibodies
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