Intercept (PBS) Blocking Buffer | LICORbio

Intercept® (PBS) Blocking Buffer

Intercept (PBS) Blocking Buffer | LICORbio

Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared (NIR) fluorescent applications when using a phosphate-buffered saline (PBS) system.

For larger pack sizes, please contact your local sales office.

Formulation

Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.

Shake well before each use.

Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye® 800CW Goat anti-Mouse IgG (P/N 925-32210) and IRDye 680LT Goat anti-Rabbit IgG (P/N 925-68021), and scanned together on an Odyssey CLx Imaging System.

Intercept (PBS) Blocking Buffer can be used for many immunoassays and applications, including:

Selected P/N: 927-70001,927-70003,927-70010,927-70025,927-70050

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*Includes all LICORBio buffers
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