IRDye 680LT Goat anti-Rat IgG Secondary Antibody

Immunogen

Rat IgG, whole molecule

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

Western Blot
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging

Not Recommended for:

Small Animal Imaging
In-Cell Western Assay
On-Cell Western Assay

Formulation

IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:10,000 - 1:50,000
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 - 0.02%) and Tween^® 20 (final concentration of 0.1 - 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

RRID

P/N 925-68029: RRID AB_2814909
P/N 926-68029: RRID AB_10715073

Example Data

IRDye® 680LT Goat anti-Rat IgG Secondary Antibody data — **Western blot detection of tubulin in C32 lysate.** Samples loaded in 4X Protein Sample Loading Buffer (P/N 928-40004), resolved on a 10% Bis-Tris gel, and transferred to Odyssey^® nitrocellulose (P/N 926-31090). The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) and probed with rat anti-tubulin (Novus Biologicals P/N NB600-506) followed by detection with IRDye 680LT Goat anti-Rat IgG (P/N 926-68029).

IRDye® 680LT Goat anti-Rat IgG Secondary Antibody