IRDye Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In Cell Western™ Assays, in vivo imaging, and whole organ or tissue section assays. Applications vary depending on the IRDye Infrared Dye selected for labeling.
IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.
IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water-soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.
For more information, refer to the IRDye Peptide Labeling Application Guide.
Special pricing is available in your location. For more information, please contact your local expert here.
| Product Name | Price |
|---|---|
IRDye® 680LT Protein Labeling Kits
The IRDye 680LT Protein Labeling Kit is optimized for labeling proteins for use with imaging on Odyssey Imagers and Pearl Imagers. |
|
IRDye® 680RD Protein Labeling Kits
The IRDye 680RD Protein Labeling Kit is optimized for labeling proteins for use with imaging on the Odyssey Family of Imagers and Pearl Imagers. |
|
IRDye® 800CW Protein Labeling Kits
IRDye 800CW Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots, In-Cell Western assays, in vivo imaging, and whole organ or tissue section assays. |